Introduction to soil nematode separation method
Soil nematodes are soil animals widely found in the soil. For better study of soil nematodes, the basic work needed in the early stage is to quickly isolate soil nematodes. Three basic soil nematode separation methods are described below.
1, Berman funnel method
This method was proposed by Baermann in 1917. The principle is that the nematode is a hi-water animal. When it meets water, it will be released from the soil or plant tissue. At the same time, it will sink into the funnel tube and the latex tube due to its own gravity. However, this method is limited to the isolation of active worm-type nematodes, which are ineffective against dead insects, inactive nematodes or insects.
2, Caber sifting method
This method was proposed by Cobb in 1918. It is mainly used to isolate nematodes in soil. The amount of samples processed is large, and the recovery rate of nematodes is high, and dead and living nematodes can be obtained at the same time. The principle of the method is that the size of the worm and the specific gravity of the nematode are lighter than water or close to water. During the panning process, the nematode can float on the surface of the water or be suspended in water. When sifting, the size of the worm can be on different meshes. Collect nematodes.
3. Centrifugal floating method
The method utilizes a sucrose solution having a relative density greater than the relative density of the nematodes to separate the nematodes from the soil or plant tissue by centrifugation. The principle is to use the difference in density to separate the nematodes from the mud and debris, especially the application of different concentrations of sucrose or other preparations, the separation effect is better. A large number of samples can be separated by generally combining the sieving method.
The above are three basic methods for the separation of soil nematodes. The separation of nematodes is the basic link and the important link in the study of nematodes. Therefore, it is very important to choose a suitable separation method. Modern, with the development of science and technology, in order to improve efficiency, enhance the effect, and separate soil nematodes, you can use a professional instrument - nematode separator to directly separate. The instrument is highly efficient and easy to use, and can help researchers quickly obtain clean nematode specimens and provide effective standard samples for subsequent identification and research work.
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