Malvern Nano Analyzers Create Perfect Tooth

Vuk Uskokovic, MD, of the Department of Oral Prevention and Rehabilitation at the University of California, San Francisco (UCSF) School of Dentistry, is working on research aimed at simulating enamel growth in the laboratory. The key analytical tool in this NIH-funded project is the Zetasizer Nano nanoparticle size and potential analysis system from Malvern Instruments. Using the instrument's comprehensive Dynamic Light Scattering (DLS) and Zeta potential measurement capabilities, Dr. Uskokovic and his colleagues were able to analyze the interaction between 90% of amelogenin, which is a glaze matrix, and the mineral component hydroxyapatite. Studies have suggested that the self-assembly of this particular protein guides the formation of enamel crystals.

Although dental techniques are often very sophisticated, the techniques that can be used to repair damaged dental tissue are often not perfect. Therefore, there is a need to develop methods that can minimize tissue damage. According to Dr. Uskokovic, if we understand the natural formation principle of enamel, we can use the same compound to remodel damaged enamel.

In a paper titled “Analysis of Zeta Potential and Particle Size of Human Amesome Proteins” (Uskokovic et al., J. Dentistry Research 89(2): 149-153, 2010), Dr. Uskokovic and his colleagues gave The results of the study, and suggested that: "Zeta potential can be used as a control parameter to reproduce the assembly of amelogenin in vitro." The authors also pointed out: "... [the article] established the correlation between Zeta potential and intergrain gravity Meaning may be universally applied to self-assembled proteins."

When asked why he chose Zetasizer Nano, a nanoanalyzer from Malvern, Dr. Uskokovic said: "I used Zetasizer Nano before, but it was used for more stable inorganic materials, so when asked to buy protein particle analysis When I was in the system, I knew it would be an ideal choice. In the parallel test with competing products, the result was not suspense. Zetasizer Nano produces results that are both reliable and stable.”

Dr. Uskokovic continued: “Proteins are very sensitive to electric fields. During the determination of Zeta potential, we all know that if we are not careful, proteins will be accidentally 'cooked', so to obtain usable results, we must Fine-tuning was done to overcome these challenges, and Malvern Instruments nanotechnology experts were able to answer questions quickly and provide detailed guidance.”

Dr. Uskokovic has completed preliminary studies covering the analysis of amelogenin, including its proteolytic cleavage products, published in the Journal of Dental Research. Next, there is an upcoming publication that focuses on a more comprehensive paper that focuses on the interaction between amelogenin and its mineral counterparts in enamel.

The Zetasizer Nano from Malvern Instruments can perform high-quality particle size and Zeta potential measurements. The reliability of the results makes this instrument ideal for protein analysis. For further information, please visit:. Com. Cn/zetasizer

Figure: Malvern Zetasizer Nano Nanoparticle Size and Zeta Potential and Molecular Weight Analyzer Malvern Instruments Ltd., the inventor of the laser particle size analyzer, is headquartered in the UK with the highest knowledge density in the city of Malvern, and the company was formerly the British National Defense Industry Laboratory. For more than 50 years since its establishment, it has always led the industry. As a universally recognized material characterization technology pioneer and industry standard, Malvern's products and technologies embody the latest achievements in molecular configuration, rheological characteristics analysis and particle characterization technology development, and are widely used in protein, pharmaceutical, petrochemical, petroleum, Ceramics, powders, coatings, cement and military and other fields. Malvern not only provides customers with products and solutions, but also emphasizes powerful service support and expertise to ensure the accuracy and efficiency of analysis and promote the development of research and production. At present, Malvern has a number of branch offices worldwide, with users in 86 countries and regions around the world. As a member of Spectris Group, Malvern Instruments has an Asia-Pacific Technology Application Laboratory and four offices in China. Its headquarter is located in Shanghai and other offices include Beijing, Guangzhou and Chengdu. . Com,. Com. Cn

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